Minimum Inhibitory Concentration Assay of Ethyl Acetate Fraction of Walay Rhizome (Meistera chinensis) Against The Inhibition of Staphylococcus aureus

Abstract

Infectious diseases are usually caused by bacteria, one of which is Staphylococcus aureus. The use of traditional medicine is currently more clinically effective because there are fewer side effects. One of the plants whose habitat is often found in South Konawe district, Southeast Sulawesi Province is walay rhizome (Meistera chinensis). This research aims to determine the minimum inhibitory concentration assay against Staphylococcus aureus bacteria and secondary metabolite compound of the ethyl acetate fraction of walay rhizome (Meistera chinensis). This research is experimental research. The sample was extracted using the maceration method using 96% ethanol, then fractionated using liquid-liquid partition using distilled water, methanol and ethyl acetate in a ratio of 1:1. The ethyl acetate fraction was separated and the minimum inhibitory concentration assay was using turbidumetry and UV-Vis Spectrophotometry methods. The data obtained was analyzed using statistical tests One Way Anova. The results of the minimum inhibitory concentration assay using the turbidumetry method at concentrations of 1.563%, 3.125%, 6.25%, 12.5%, 25% and 50% were clearer than the negative control tube. In the test using the Uv-Vis Spectrophotometry method, the smallest concentration of 1.563% to the largest concentration of 50% showed a decrease in the absorbance value. Results of phytochemical screening of the ethyl acetate fraction of walay rhizome (Meistera chinensis) positively compounds such as flavonoid, tannins and saponins as antibacterial compounds.