Formulation and Antioxidant Activity of Cream Marine Sponge Extract (Axinella carteri) by Using the DPPH Method (1.1-Diphenyl-2-Picrylhydrazyl)

Abstract

Sponges are one of the marine biotas that has bioactive potential that is useful as an antioxidant to prevent free radicals. This study aims to find out of preparations antioxidant activity cream containing marine sponge extracts (Axinella carteri) against DPPH and how much concentration of a cream extract marine sponge (Axinella carteri) that has the highest activity rate as antioxidant. The marine sponges are extracted by maceration method using methanol solvents then made preparations in cream form using variations in extracts concentrations of 5%, 7,5% and 10%. Furthermore, the cream formulation of marine sponge extract was tested for the preliminary cream testing parameters like organoleptic test, homogeneity, spreadability, pH, viscosity and cream type test. Antioxidant activity determined with a method of DPPH that expressed in IC50 (Half-maximal inhibitory concentration). The result of IC50 obtained in formula I is 539,74 ppm (very low), formula II is 398,00 ppm (low) and formula III is 244,14 ppm (medium). Meanwhile, for the positive control vitamin C has an IC50 value of 5.02 ppm (very active). Formula III is the best formula which has medium rate of antioxidant activity.