Acute Toxicity Test of Ethanol Extract of Sapodilla Stem Bark (Manilkara zapota (L.)) by BSLT Methods
Keywords:
Sapodilla bark (Manilkara zapota (L.)), Acute toxicity, BSLT (Brine Shrimp Lethality Test)Abstract
Sapodilla (Manilkara zapota (L.)) is one of the most widely grown plants in West Kalimantan. Sapodilla stem bark contains secondary metabolites of flavonoids and saponins, which have toxicity activity so that it is expected to be an alternative for cancer treatment. The purpose of this study was to determine whether the ethanol extract of sapodilla stem bark (Manilkara zapota (L.)) is toxic and what is the LC50 value produced by the BSLT. This study used the ethanol extract of sapodilla stem bark with various concentrations of 0 µg / ml; 100 µg / ml; 250 µg / ml; 500 µg / ml; 750 µg / ml; 1000 µg / ml with 5 repetitions and each concentration consisted of 10 larvae of Artemia salina Leach. Data on larvae mortality were collected and analyzed using the probit analysis method. Based on the regression results, the LC50 value calculated manually or using Microsoft Excel is 190,54 µg /
ml. This study indicates that ethanol extract of sapodilla stem bark is cytotoxic against the larvae of Artemia salina Leach
