Antibacterial Activity Ethanol Extract of Chinese Petai (Leucaena leucocephal (Lam.De Wit), guava leaves ( Psidium guajava L.) and their combinations on the growth of Escherichia coli

Abstract

Chinese petai leaf (Leucaena Leucocephal (Lam.) de wit , Guava leaf (Psidium guajava L.) is one of the natural ingredients that can be used as an antibacterial. This study aims to determine the class of secondary metabolite compounds and antibacterial activity against Escherichia coli bacteria by pouring and paper disc diffusion methods. Simplified Chinese petai leaf (Leucaena Leucocephal (Lam.) de guava leaves (Psidium guajava L.), and their combination macerated with 70% ethanol. Phytochemical Screening Results of ethanol extract Chinese petai leaf (Leucaena Leucocephal (Lam.) de wit contain tannins, alkaloids, saponins, and flavonoids, Guava leaf (Psidium Guajava L.) contain alkaloids, tannins, saponins, and flavonoids. Furthermore, TLC was carried out to confirm the presence of chemical compounds in the extracts of Chinese petai leaf (Leucaena Leucocephal (Lam.) de wit, guava leaf (Psidium Guajava L.) and their combination using the mobile phase N-Hexane: Ethyl Acetate (4:1:5). ) with silica gel GF254 a stationary phase with 5% FeCl3 spotting for phenolics and 5% AlCl3 for flavonoids then detected at 254 nm and 366 nm UV light. The positive control used Co-trimoxazole 50 g while the negative control was physiological NaCl. Based on the results, the average diameter of the inhibition zone of the ethanolic extract of Chinese petai leaf (Leucaena Leucocephal (Lam.) de wit against Escherichia coli bacteria with a concentration of 25 mg/mL, 20 mg/mL 15 mg/mL was 11.04 mm; 9.32 mm; 7.82 mm, guava leaf ethanol extract (Psidium guajava L.) against Escherichia coli bacteria with a concentration of 25 mg/mL, 20 mg/mL 15 mg/mL, namely 13.94 mm; 9.88mm; 8.7 mm and ethanol extract combination of Chinese petai leaf (Leucaena Leucocephal (Lam.) de wit and guava leaf (Psidium Guajava L.) with a concentration of 25 mg/mL, 20 mg/mL 15 mg/mL, namely 14.98 mm ; 12.96mm; 11.46mm. Based on the results of the One Way Anova test, it showed the effect of antibacterial activity on Escherichia coli with a
significant value of 0.00<0.05. This indicates that the ethanol extract has activity on Gram-positive.